Improved Culture Flask for Obligate Anaerobes

Improved culture flask for obligate anaerobes.

An improved flask system for the growth of extremely oxygen-sensitive bacteria in liquid culture is described. The improvement described utilizes an all-glass, neoprene-stoppered flask designed for growth of 50- to 1,000-ml cultures of bacteria with continuous gassing.

Obligate anaerobes which form skatole.

Although the presence of skatole in decomposing protein materials and in feces has frequently been reported, very rarely have microorganisms been shown to form skatole in pure culture. Two species of the genus Clostridium have been described as producing skatole: Clostridium skatol Fellers and Clough (J. Bacteriol., 10, 130, 1925), which had been characterized earlier by Fellers (Abstr. Bacteri...

Pipette count method for obligate anaerobes.

The routine counting of obligate anaerobic bacteria is a serious problem in many laboratories. Reliable anaerobic chambers are either expensive or messy and cumbersome. Furthermore, the exposure of certain genera, notably Bacteroides and some spirochetes, to air may kill the organisms. The following technique does not require an anaerobic chamber, does not expose the organism to atmospheric oxy...

Dialysis Flask for Concentrated Culture of Microorganisms.

Gerhardt, Philipp (The University of Michigan, Ann Arbor), and D. M. Gallup. Dialysis flask for concentrated culture of microorganisms. J. Bacteriol 86:919-929. 1963.-A twin-chambered dialysis flask was designed with a supported membrane clamped between a reservoir of medium in the bottom and a small volume of culture above, the unit being mounted on a shaking machine to provide aeration and ag...

Self-contained culture flask system.